Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 1M MES, pH 5.76. 1000mL, Sterile 2 pack.
MES solution is a zwitterionic buffer used to help maintain pH levels in biochemistry experiments. It has a buffering range of pH 5.8 - 6.5.
Teknova 1M Ammonium Sulfate, 10mM Tris-HCl pH 8.0. 500mL, Sterile. 4 Pack.
1M Ammonium Sulfate,10mM Tris-HCl
Axxora LLC Hepes Buffer 1 M pH 7.5
Hepes Buffer is one of the best general purpose buffers available for biological research. It has been used to advantage in tissue culture, oxidative phosphorylation and protein synthesis with cell-free bacterial systems. Hepes is a recommended buffer for the glutamate binding assay, prevents binding to non-receptor materials.
Newcomer Supply Phosphate Buffer 0.1M, pH 7.2 to 7.4, 1 gallon
Use: IHC wash and rinse. Shelf Life is 1 year from date of manufacture. Store at 2-8°C.
Chem Service Inc Tris(1,3-dichloroisopropyl)phosphate | 13674-87-8 | MFCD00083121 | 250mg
Tris(1,3-dichloroisopropyl)phosphate | MW: 430.88 | 13674-87-8 | MFCD00083121 | 250mg
PROVISION KINETICS INC TRIS- SALINE BUFFER
500 ml bottle of Tris-saline buffer, pH 8.4 designed for use with Anti-IIa and Anti-Xa heparin test kits (sold separately)
Bioworld PIPES BUFFER 1M PH 6.0 100ML
liquid, 1.0m, ph 6Synonym(s): 1,4-Piperazinediethanesulfonic acidMolecular Formula: C8H18N2O6S2CAS Number: 5625-37-6Molecular Weight: 302.37
Andwin Scientific VWR PBS-T BUFFER POWDER 100/CS
VWR® Buffer Powder Packs can save time and money, as there is no need to measure and weight many separate chemical components which is required when making buffers from scratch.Buffer powder quickly dissolves to make 1L of 1X solutionNo weighing or measuring of separate componentsNo pH adjustments requiredEconomical shipping, long shelf lifeQuality and consistency guaranteedVWR® Pre-measured buffer packs are extremely easy to use and are formulated so each pack makes 1 Liter of 1X buffer. Simply tear open a pack and mix completely with 1 liter of distilled water.Shipping costs are also minimal, as each case of 50 or 100 packs is compact and weighs only 2.5 Lbs.
Bioworld BES-Buffered Saline (2X), 250 mL
Product DescriptionBES (N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) saline buffer has a concentration of 2X and a pH of 7.1 at 25°C. BES is a zwitterionic buffer used in biochemistry and molecular biology, and has been used to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids. BES was used to determine that saline buffers minimize the action of interfering factors in the bacterial endotoxins test. This saline buffer contains 50 mM BES, 1.5 mM Na2HPO4, and 280 mM NaCl.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Hampton Research HR2-723/1.0 M BICINE pH 9.0 - 100 ml
HR2-723/1.0 M BICINE pH 9.0 - 100 ml. Please note items from this supplier are non-returnable.